Photodynamic Action of Proflavine on Coliphage T3 I. Kinetics of Inactivation

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Photodynamic action of proflavine on coliphage T3. I. Kinetics of inactivation.

The photodynamic inactivation of coliphage T3 was studied over a wide range of concentrations of the dye proflavine. With 2 x 10(7) phage/ml, two modes of inactivation were observed. Between 0.25 and 12 to 13 mug/ml, inactivation was biphasic. There was an initial first-order inactivation (Rx1) which became temporally associated with an apparently multiorder process (Rx2) at higher light doses....

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On the mechanism of the acridine orange sensitized photodynamic inactivation of lysozyme. I. Basic kinetics.

The kinetics of the photodynamic desactivation of lysozyme in presence of acridine orange as the sensitizer have been investigated in detail varying oxygen, protein, dye concentration, ionic strength and pH value. The kinetics can be approximately described as an over all pseudo-first-order rate process. Changing the solvent from water to D2O or by quenching experiments in presence of azide ion...

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Survival of T3 coliphage in varied extracellular environments. I. Viability of the coliphage during storage and in aerosols.

The objective of this study was to determine the feasibility of using airborne T3 coliphage as a viral tracer in microbial aerosols. Although T3 coliphage was relatively stable when stored either at temperatures ranging from 21 to 37 C or in the frozen state at -20 C, there was a 2-log loss in infectivity when stored for 72 days at 4 C. Either agitation of stored coliphage suspensions held at 3...

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Photosensitization of T2 coliphage with toluidine blue.

The photodynamic action of dyes upon biological systems has been reviewed by Blum (1941). Inactivation of bacteriophage by this process has been studied by Clifton (1931), Perdrau and Todd (1933), Burnet (1933), Morales (1941), and Pacheco and Morales (1941). More recently, Welsh and Adams (1954) and Yamamoto (1956, 1958) investigated the kinetics of photodynamic inactivation of coliphage. In t...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1971

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.7.3.314-318.1971